Coding

Part:BBa_K3504016:Design

Designed by: Ahmed Wael   Group: iGEM20_AFCM-Egypt   (2020-10-15)


nSP4-RNA-directed RNA Polymerase-Semliki forest virus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1101
    Illegal PstI site found at 832
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 594
    Illegal PstI site found at 832
    Illegal NotI site found at 598
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1101
    Illegal PstI site found at 832
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1101
    Illegal PstI site found at 832
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1091


Design Notes

NA


Source

IDT

References